Mice can teach us about human disease

Scientists understand what fewer than one-fifth of human genes do; probing mice can help fill the gaps

Zorana Berberovic gently lifts a small black mouse by its tail. As its hind legs rise up off the floor of its cage, the research technician slips a tiny vial under the mouse’s bottom. Berberovic lightly strokes her gloved finger against its belly. Within seconds, she is rewarded. A dribble of pee enters the vial.
“They have small bladders so there’s not much,” Berberovic says. Luckily, she adds, “We don’t need much.”
It’s hard to imagine that someone might need any mouse pee at all. But there could be a lot to learn from the urine of this particular mouse. It could help scientists better understand important aspects of human disease.
Berberovic works at the Toronto Centre for Phenogenomics (FEE-no-geh-NO-miks) in Toronto, Canada.
Wait: pheno-what?
Pheno is a prefix that comes from the Greek word meaning “to show.” Biologists often borrow this prefix to explain the basic traits of an organism: its phenotype. A mouse tends to be small, furry, four-footed and shy with a long, naked tail. Those descriptions are all part of its phenotype. Meanwhile, genomics is the study of the genetic material an organism has inherited from its parents. When combined, these two terms describe the study of how an organism’s genome contributes to its phenotype — or those traits we can observe.
The Toronto center is one of 18 institutes around the world that make up the International Mouse Phenotyping Consortium. Scientists at these institutes are working together to figure out the function of every mouse gene.
A gene is a segment of DNA that influences how an organism looks and functions. While humans and mice look and act very differently, 85 to 90 percent of our genes are the same or at least very similar. So by understanding the instructions in every mouse gene, people should get a pretty good idea of the instructions in virtually every human gene too.
But making a link between mouse and human genes is not always easy. While the genes are indeed quite similar, humans don’t normally have tails or whiskers. This is because the areas of DNA on either side of a human or mouse gene are sometimes different. These parts of the DNA are called control regions. Such regions can change the way a gene’s instructions get carried out.
Berberovic and her fellow researchers even want to know which genes affect pee. They especially want to know whether chemicals that the body dumps into urine can tell us how healthy — or sick — an individual might be.
But the consortium’s many scientists are looking well beyond pee. Their research may tease out which genes affect an animal’s size, weight, behavior — even lifespan. Matching a gene with the effect it has on those characteristics or traits is called phenotyping.
Ann Flenniken is a molecular geneticist at the Toronto center. (It’s run jointly by Mount Sinai Hospital and The Hospital for Sick Children, both in Toronto.) She studies what genes do and the chemical basis for those functions.
By working together, she says, the global phenotyping consortium hopes one day to amass “a catalogue of all gene functions.”

So many genes, so little information
The cells in all living things contain genes. They’re made from DNA. And those genes instruct cells about what to do as they develop, grow, interact with their neighbors — and ultimately die. Some instructions might guide an organism’s vision or its hearing, Flenniken points out. They might determine its reproductive habits or even the way the animal walks. You might think of a gene as a blueprint for a certain cellular task.
Proteins are what carry out those tasks. So a gene’s DNA really provides instructions for making proteins, Flenniken explains. Proteins are an essential part of all living organisms. They form the basis of living cells and tissues (like brain, muscle and skin). But just as importantly, proteins do the work inside of cells.
Both humans and mice have roughly 20,000 genes. Right now, scientists understand the function of fewer than one out of every five of those genes.
That’s frustrating for someone like Jim Woodgett. He is a cell biologist and the director of research at Toronto’s Mount Sinai Hospital.
“We don’t know about 80 percent of the instructions for life. That’s unbelievable,” Woodgett says. “It’s essential that we learn what genes do.”
Mechanics can’t fix cars if they don’t know how each part works, he notes. Similarly, doctors can’t treat human diseases if they don’t know how the genes that play a role in those ailments work.

Looking for defects as a clue to treatments
Woodgett studies causes and treatments for a wide range of illnesses. These include cancer, diabetes, Alzheimer’s disease and bipolar disorder. (That last one is a mental illness characterized by alternating periods of extreme depression and extreme joy.)
These illnesses have very different symptoms. Yet they all have something important in common: genes that don’t contain the right instructions to make proteins work properly.
Perhaps a gene normally produces a protein that stops cancer cells from growing. Someone who inherits a faulty copy of that gene might face a higher than normal risk of getting cancer.
But if scientists could identify that gene, they might be able to create a medicine that overcomes the defect. An example might be a drug that contains the very protein that the faulty gene was supposed to make.
Yet until scientists such as Woodgett learn how most genes work, such treatments will remain only a dream.

Ka-pow! Knocking out genes
Researchers with the International Mouse Phenotyping Consortium are looking for answers. In fact, they have already made an important discovery at the Wellcome Trust Sanger Institute, in Cambridge, England.
In 2012, researchers there found nine mouse genes that instruct proteins on how to give bones strength and flexibility. Now other medical researchers are hoping to come up with treatments for people with bone diseases that might be caused by problems with one of these nine genes.
The institute’s researchers discovered what each gene did by first turning it off and then observing what happened. In fact, all researchers in the phenotyping consortium use mice with inactivated genes.
“We turn off one gene at a time,” explains geneticist Jacqueline White. She is the lead manager for mouse phenotyping at the institute. All remaining genes in the animals continue to work normally. Consortium scientists “then all run the same tests on the mice,” she explains. These may include tests on their hearing, vision, urine and blood — even how easily the mice become startled.
When scientists turn off a gene’s instructions, they “knock out” its function. So scientists refer to a mouse with an inactivated gene as a “knock-out mouse.”
Here’s how they create such an animal:
Genes are made of two intertwined strands of DNA. Ladder-like rungs link each strand to its partner. Each rung on the ladder is made from two chemicals known as nucleotides.
Nucleotides come in four types: adenine (abbreviated A), thymine (T), cytosine (C) and guanine (G). T links only with A; C links only with G.
Instructions for making proteins are determined by the order of those nucleotide pairs in a gene. Changing their order changes the instructions they will later give to cells. Scientists can deliberately change the order of nucleotides in a gene so that its recipe no longer makes its intended protein. Doing this turns off — or knocks out — the gene.
Sometimes the switched-off gene is so important that a developing mouse won’t survive to birth. Indeed, “about 20 to 30 percent of the genes we knock out end up causing mice not to survive,” says Kent Lloyd. He’s a veterinary scientist at the University of California, Davis. That university is part of the International Mouse Phenotyping Consortium.
When this prenatal death occurs, “Often it’s because the gene we knocked out affects the heart,” Lloyd says. “If the heart doesn’t work, the animal dies.”

Engineering the right stuff in your mouse
Many human embryos don’t survive. Scientists would like to know whether genes might underlie the problem (as opposed to trauma or infection, for instance). That’s another question the consortium researchers hope to tease out with the help of their mice.
But not just any mouse will do. All consortium scientists use a special strain of mouse. A strain is a group of animals belonging to the same species that are very similar genetically. All the mice of a certain strain might have the same color coat or might all get fat on a low-fat diet. They might all develop diabetes, cancer — or even dementia or the mouse equivalent of a low IQ. Scientists create these strains by crossbreeding animals with a particular set of genes again and again, over many generations. In the end, these mice become almost like twins — or certainly very, very close cousins of each other.
In the wild, a gene can vary quite a bit from mouse to mouse. But in a strain of mice, all of the genes are very similar. This consistency is important.
The researchers want to make sure that small differences in gene types don’t affect test results. They want to know the only thing affecting the way the animal’s body looks or functions is the presence (or absence) of a particular working gene.
Inbreeding to quickly and efficiently isolate desired genetic traits would be much harder in humans. We have longer lives and take much longer to reproduce than mice do. More importantly, breeding people for research would violate many beliefs, values and laws.
That is why these furry mammals are the subjects of so many experiments — experiments ultimately meant to tease out the mysteries of human genes.
All scientists in the global phenotyping consortium use a strain of mouse called C57BL/6. Although black, these mice otherwise resemble normal field mice. What is really different about them isn’t visible to the eye: The genes in every member of this strain are almost totally identical.
While these mice have proven useful, consortium scientists use as few animals as possible in their experiments. “We don’t use animals like we use a chemical off a shelf,” says Lloyd. “They are living creatures.”
A special committee of scientists and non-scientists also oversees every experiment to make sure that the scientists treat their mice as humanely as possible.
For example, mice are kept in groups of up to five in a cage. That is because they prefer the company of other mice. Researchers feed the animals regularly and give them exercise wheels or toys to stimulate their minds. They provide the mice comfortable bedding material to make nests, and replace that bedding when urine and feces soil it. Scientists also ensure the mice are protected from predators and infections.
Observes Flenniken, “These animals suffer far less in our facility than they would in the outside world.”
Still, she and other scientists understand why people may be uncomfortable with performing experiments on animals. “There are ethical and moral issues to using animals in research, especially when we are the beneficiaries,” says Woodgett. However, he adds: “The alternative is to say ‘Let’s test this not on a mouse, but on you.'”

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